Platelet Rich Plasma Injection: Difference between revisions
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(Created page with "Procedure described for doing one leukocyte rich and one leukocyte poor injection. ==Equipment== *Syringes **60mL syringe for the blood **10mL syringe for the local anaesthet...") |
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*3 x ACDA tubes for the anticoagulant | *3 x ACDA tubes for the anticoagulant | ||
*PRP kit | *PRP kit | ||
**One cartridge for the blood | **One cartridge for the blood (e.g. omni PRP which is ~6x concentration, or regen which is ~2x concentration) | ||
**Another cartridge as a counterbalance, measured to weigh the same, filled with water. | **Another cartridge as a counterbalance, measured to weigh the same, filled with water. | ||
*Centrifuge | *Centrifuge | ||
| Line 21: | Line 21: | ||
*PRP benchtop processing station. | *PRP benchtop processing station. | ||
== | ==Preparation== | ||
*Extract ~2.5mL of ACDA into the 60mL syringe | *Extract ~2.5mL of ACDA into the 60mL syringe | ||
*Draw up 55mL of blood into the 60mL syringe | *Draw up 55mL of blood into the 60mL syringe | ||
| Line 27: | Line 27: | ||
*Place the cartridge into the centrifuge, along with an equal counterweight cartridge. | *Place the cartridge into the centrifuge, along with an equal counterweight cartridge. | ||
*Spin at 3350rpm for 7 minutes | *Spin at 3350rpm for 7 minutes | ||
*Remove the cartridge, it should be separated into | *Remove the cartridge, it should be separated into red cells at the bottom, leukocytes in the middle, and plasma at the top | ||
*Place the cartridge into the PRP benchtop processing station, facing up | *Place the cartridge into the PRP benchtop processing station, facing up | ||
*Use the dial on the station to control the plunger | *Use the dial on the station to control the plunger | ||
| Line 34: | Line 34: | ||
*Transfer the top remaining half (leukocyte poor) into a 10mL syringe | *Transfer the top remaining half (leukocyte poor) into a 10mL syringe | ||
*Transfer the bottom remaining half, including some of the leukocytes into another 10mL syringe. | *Transfer the bottom remaining half, including some of the leukocytes into another 10mL syringe. | ||
==Procedure== | |||
;Joint | |||
*Infiltrate the skin and capsule with local anaesthetic but not the joint space | |||
*Inject into the joint space or joint structure with the leukocyte poor solution | |||
;Tendon | |||
*Inject around the tendon with the leukocyte rich structure | |||
==Aftercare== | |||
Leukocyte rich PRP can cause a pain flare for a few days. Advise to wait for minimum 4 weeks before assessing efficacy. | |||
[[Category:General Procedure Concepts]] | [[Category:General Procedure Concepts]] | ||
Revision as of 18:04, 5 August 2020
Procedure described for doing one leukocyte rich and one leukocyte poor injection.
Equipment
- Syringes
- 60mL syringe for the blood
- 10mL syringe for the local anaesthetic
- 10mL syringe for the leukocyte poor PRP
- 10mL syringe for the leukocyte rich PRP
- Needles
- butterfly set for taking blood
- drawing up needle
- 25g needle for the local anaesthetic
- Needle for the PRP injection e.g. 22g 70mm spinal needle for the hip joint.
- 3 x ACDA tubes for the anticoagulant
- PRP kit
- One cartridge for the blood (e.g. omni PRP which is ~6x concentration, or regen which is ~2x concentration)
- Another cartridge as a counterbalance, measured to weigh the same, filled with water.
- Centrifuge
- Sterile ultrasound jelly
- Ultrasound machine
- PRP benchtop processing station.
Preparation
- Extract ~2.5mL of ACDA into the 60mL syringe
- Draw up 55mL of blood into the 60mL syringe
- Place the drawing up syringe onto the syringe, open the top of the cartridge, and then transfer the mixed blood/ACDA solution. Close the top
- Place the cartridge into the centrifuge, along with an equal counterweight cartridge.
- Spin at 3350rpm for 7 minutes
- Remove the cartridge, it should be separated into red cells at the bottom, leukocytes in the middle, and plasma at the top
- Place the cartridge into the PRP benchtop processing station, facing up
- Use the dial on the station to control the plunger
- Remove and discard the top half of the plasma
- The remaining plasma is leukocyte poor at the top, and leukocyte rich at the bottom. The very bottom of the plasma has the leukocytes.
- Transfer the top remaining half (leukocyte poor) into a 10mL syringe
- Transfer the bottom remaining half, including some of the leukocytes into another 10mL syringe.
Procedure
- Joint
- Infiltrate the skin and capsule with local anaesthetic but not the joint space
- Inject into the joint space or joint structure with the leukocyte poor solution
- Tendon
- Inject around the tendon with the leukocyte rich structure
Aftercare
Leukocyte rich PRP can cause a pain flare for a few days. Advise to wait for minimum 4 weeks before assessing efficacy.
