Platelet Rich Plasma Injection
From WikiMSK
Procedure described for doing one leukocyte rich and one leukocyte poor injection.
Equipment
- Syringes
- 60mL syringe for the blood
- 10mL syringe for the local anaesthetic
- 10mL syringe for the leukocyte poor PRP
- 10mL syringe for the leukocyte rich PRP
- Needles
- butterfly set for taking blood
- drawing up needle
- 25g needle for the local anaesthetic
- Needle for the PRP injection e.g. 22g 70mm spinal needle for the hip joint.
- 3 x ACDA tubes for the anticoagulant
- PRP kit
- One cartridge for the blood
- Another cartridge as a counterbalance, measured to weigh the same, filled with water.
- Centrifuge
- Sterile ultrasound jelly
- Ultrasound machine
- PRP benchtop processing station.
Procedure
- Extract ~2.5mL of ACDA into the 60mL syringe
- Draw up 55mL of blood into the 60mL syringe
- Place the drawing up syringe onto the syringe, open the top of the cartridge, and then transfer the mixed blood/ACDA solution. Close the top
- Place the cartridge into the centrifuge, along with an equal counterweight cartridge.
- Spin at 3350rpm for 7 minutes
- Remove the cartridge, it should be separated into blood at the bottom, and plasma at the top
- Place the cartridge into the PRP benchtop processing station, facing up
- Use the dial on the station to control the plunger
- Remove and discard the top half of the plasma
- The remaining plasma is leukocyte poor at the top, and leukocyte rich at the bottom. The very bottom of the plasma has the leukocytes.
- Transfer the top remaining half (leukocyte poor) into a 10mL syringe
- Transfer the bottom remaining half, including some of the leukocytes into another 10mL syringe.