Platelet Rich Plasma Injection

Procedure described for doing one leukocyte rich and one leukocyte poor injection.

Equipment

Syringes
- 60mL syringe for the blood
- 10mL syringe for the local anaesthetic
- 10mL syringe for the leukocyte poor PRP
- 10mL syringe for the leukocyte rich PRP
Needles
- butterfly set for taking blood
- drawing up needle
- 25g needle for the local anaesthetic
- Needle for the PRP injection e.g. 22g 70mm spinal needle for the hip joint.
3 x ACDA tubes for the anticoagulant
PRP kit
- One cartridge for the blood
- Another cartridge as a counterbalance, measured to weigh the same, filled with water.
Centrifuge
Sterile ultrasound jelly
Ultrasound machine
PRP benchtop processing station.

Extract ~2.5mL of ACDA into the 60mL syringe
Draw up 55mL of blood into the 60mL syringe
Place the drawing up syringe onto the syringe, open the top of the cartridge, and then transfer the mixed blood/ACDA solution. Close the top
Place the cartridge into the centrifuge, along with an equal counterweight cartridge.
Spin at 3350rpm for 7 minutes
Remove the cartridge, it should be separated into blood at the bottom, and plasma at the top
Place the cartridge into the PRP benchtop processing station, facing up
Use the dial on the station to control the plunger
Remove and discard the top half of the plasma
The remaining plasma is leukocyte poor at the top, and leukocyte rich at the bottom. The very bottom of the plasma has the leukocytes.
Transfer the top remaining half (leukocyte poor) into a 10mL syringe
Transfer the bottom remaining half, including some of the leukocytes into another 10mL syringe.